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Automated cell culture

Trilobio automates media exchange, feeding, and passaging on a fixed schedule, so your cells get maintained on time whether or not anyone's in the lab.

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Cell culture automation on Trilobots

Cell culture runs on a long clock. Cultures grow, get fed, and get tested over days or weeks, and someone typically has to be in the lab on a strict schedule no matter what day it lands on. The Trilobot takes over that core liquid handling itself, from feeding to swapping out spent media. Whatever your process looks like, the Trilobio platform is built with flexibility to support it.

Automated cell culture workflow diagram

Start small, scale when you’re ready

Trilobio scales with your lab. Start with a single Trilobot and add capacity as your cell culture workflows grow, without re-architecting your process. Because the platform is flexible by design, the same system that maintains your cultures today can pick up new steps (a different media formula, a new feeding interval, even a different assay entirely) as your process changes.

One Trilobot Two Trilobots Three Trilobots
Best for Targeted solution for cell handling and assay prep tasks Labs seeking high walkaway time for colony maintenance Labs optimizing for high-throughput operations
What it automates ~12% of your cell culture workflow ~80% of your cell culture workflow ~96% of your cell culture workflow
Integrated equipment None 1-2 frames with multiple instruments 3-4 frames with multiple instruments
Throughput 1000+ samples/day 1000+ samples/day 2000+ samples/day
Walkaway time* 3 hours 19 hours 23 hours
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*Based on a 24-hour workflow

At $25k/year, the Trilobot pays for itself

Traditional lab automation systems can run into six figures. Learn how you can automate cell culture in your lab at a fraction of the cost.

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What is cell culture?

Cell culture is the foundation almost every drug discovery workflow is built on, whether you're running passaging cycles to keep a line alive or testing drug efficacy against a growing population, you need cells, and you need them maintained reliably. The core building blocks are largely the same across labs: bacterial and mammalian cells, core human cell types, and (increasingly) stem cell lines, whether grown adherent on a plate or suspended in liquid, each with its own media and handling requirements. Cell culture is a "hurry up and wait" process, where the real time spent isn't on a single pipetting step, but rather keeping a strict maintenance schedule over days or weeks. Manual processes can break down (for example, a missed feeding, a late media exchange, or an inconsistent hand-off between passages) and can threaten a culture. Cell culture automation matters more for reliability and walkaway time than raw throughput. That's the workflow Trilobio automates.

Challenges

Challenges of manual cell culture

Waiting Between Steps

Between timed incubations, washes, and spin steps, there's plenty of downtime—but you still can't leave the bench. You're present for the whole run, productive for only part of it.

Equipment Babysitting

Multi-step prep requires constant attention across timed incubations, reagent additions, and plate transfers, keeping a technician tethered to the bench for most of the run.

Low Throughput

Preparing samples manually can consume an entire day of technician time.

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Poor Sample-to-Sample Consistency

Variability between technicians and across runs adds noise to your data.

Opportunity Cost

Sample prep isn’t where the innovation happens, but it's often where scientists spend their time.

benefits

Benefits of cell culture automation

Built-In Reliability

Consistent liquid handling at every step means data quality reflects your biology, not variability introduced at the bench.

Full Walkaway

Load your samples and walk away. Trilobot runs multi-step protocols unattended—no babysitting required.

More Data Points Per Plate

Because the system is integratable with your assay setup, every well can run a different condition instead of duplicating one, giving you more usable data out of each plate.

Lower Contamination Risk

Every Trilobiot is enclosed and HEPA-filtered, keeping your samples sterile from start to finish.

End-to-End on One Platform

More of your workflow runs on one platform, so you spend less time managing instrument handoffs and more time on the science.

Run-to-Run Reproducibility

Every sample follows the same protocol to the same specification—no operator variation between technicians or across runs.

Flexible by Design

Swap tools, scale volumes, change formats—the Trilobot adapts to your workflow, not the other way around.

Starts at $25k/year

Full lab automation starting at $25k/year—less than most labs spend on a single liquid handler.

Ready to automate cell culture in your lab?

Tell us about your cell culture workflow, and we'll show you exactly how the Trilobot fits in.
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Frequently asked
questions

Explore how automated cell culture works, what makes it different with the Trilobot, and how Trilobio transforms everyday biology workflows.

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How much does automated cell culture cost?

Automating cell culture starts at $25k/year with Trilobio.

Which cell lines does Trilobio support?

Trilobio works with both adherent and suspended cell cultures. Whichever you're running, we can help configure the right equipment for your incubation and assay needs.

Can I collect environmental data on my cell culture platform?

Yes. The Trilobot integrates with temperature, humidity, and particle sensors through direct API support, which is especially useful if you're building toward a self-driving lab or feeding conditions into an AI-driven workflow.

Which assays or detection methods do you support?

The Trilobot integrates with high-content imaging, automated microscopy, structural assays, and nucleic acid extraction for genome and transcriptome analysis, along with most other automation-friendly lab devices.

Do I need to write custom scripts to set up my cell culture protocol?

No. Protocols are designed in Trilobio OS using biology-level language—you define the steps the way a biologist would describe them, not in robotics code. Our team also provides onboarding support to get your specific protocol running quickly.

Powerful and reliable.
Full lab automation
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