Automated PCR

Automate PCR master mix prep and plate setup on the Trilobot, then run thermal cycling on any integrated cycler for consistent, hands-off runs.

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Automating PCR on Trilobots

PCR runs in two steps: preparing your mastermix and laying out reactions, then thermal cycling. The Trilobot handles mastermix prep and full plate layout on deck, then runs the thermal cycling step through your cycler, on-deck or off-deck, start to finish.

A diagram showing how an automated PCR workflow works

At $25k/year, the Trilobot pays for itself

Traditional lab automation systems can run into six figures. Learn how you can automate PCR in your lab at a fraction of the cost.

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What is PCR?

Polymerase Chain Reaction (or more commonly known as PCR) is used to amplify a specific DNA sequence into millions of copies, using a thermal cycler to repeatedly heat and cool a reaction mix of primers, polymerase, and template DNA. It's a core technique in molecular biology labs, used for genotyping, cloning verification, pathogen detection, and diagnostic validation.

Challenges

Challenges of manual PCR

Human Error

With so many variables to account for, even a skilled, careful technician can't always run the same protocol exactly the same way twice.

Low Throughput

Preparing samples manually can consume an entire day of technician time.

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Poor Sample-to-Sample Consistency

Variability between technicians and across runs adds noise to your data.

Equipment Babysitting

Multi-step prep requires constant attention across timed incubations, reagent additions, and plate transfers, keeping a technician tethered to the bench for most of the run.

Opportunity Cost

Sample prep isn’t where the innovation happens, but it's often where scientists spend their time.

benefits

Benefits of automating PCR

End-to-End on One Platform

More of your workflow runs on one platform, so you spend less time managing instrument handoffs and more time on the science.

Run-to-Run Reproducibility

Every sample follows the same protocol to the same specification—no operator variation between technicians or across runs.

Built-In Reliability

Consistent liquid handling at every step means data quality reflects your biology, not variability introduced at the bench.

Full Walkaway

Load your samples and walk away. Trilobot runs multi-step protocols unattended—no babysitting required.

More Data Points Per Plate

Because the system is integratable with your assay setup, every well can run a different condition instead of duplicating one, giving you more usable data out of each plate.

Flexible by Design

Swap tools, scale volumes, change formats—the Trilobot adapts to your workflow, not the other way around.

Starts at $25k/year

Full lab automation starting at $25k/year—less than most labs spend on a single liquid handler.

Ready to automate PCR in your lab?

Tell us about your PCR workflow, and we'll show you exactly how the Trilobot fits in.
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Frequently asked
questions

Explore how PCR automation works, what makes it different with the Trilobot, and how Trilobio transforms everyday biology workflows.

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How expensive is it to automate PCR?
What diseases can PCR detect?
What are the benefits of automating PCR?
How does the Trilobot compare to other PCR automation systems?
What are the key steps to automating PCR?