Next Generation Sequencing (NGS) Automation
From library prep to sample normalization, the Trilobot handles the repetitive, error-prone steps that stand between your samples and sequencing-ready plates.
Automating NGS on Trilobots
A full NGS library prep runs 5-8 hours from input to sequencing-ready output. The Trilobot is built to automate your NGS workflow, scale throughput, and increase walkaway time. No babysitting required.

Start Small. Scale When You're Ready.
We get it—you may not need a full automation buildout on day one. Start with a single Trilobot, prove out your process, and expand when your throughput demands it—without rebuilding your protocol or your setup from scratch.
What is Next-Generation Sequencing?
Next-generation sequencing (NGS) is a massively parallel sequencing technology that generates high-resolution genomic data across entire genomes, transcriptomes, or targeted gene panels in a single run. It's used across academic research, pharma, and biotech to characterize genetic variation, profile gene expression, identify somatic mutations, and map epigenetic modifications, producing comprehensive data that manual sequencing methods can’t deliver at scale. The sequencing itself is the easy part. Library preparation—the fragmentation, end repair, adapter ligation, cleanup, and normalization steps that happen before your sample reaches the instrument—is where precision matters most and where manual pipetting introduces the most risk. A small volume error or an inconsistent bead cleanup can skew coverage, introduce artifacts, or invalidate samples entirely. That's the workflow Trilobio automates.
Challenges of Manual NGS Library Prep
Benefits of Automating Next Gen Sequencing with Trilobio
Built-In Reliability
Consistent liquid handling at every step means data quality reflects your biology, not variability introduced at the bench.
Full Walkaway
Load your samples and walk away. Trilobot runs multi-step protocols unattended—no babysitting required.
More Samples, Less Time
The Trilobot runs unattended through multi-step protocols, reducing hands-on time across high-volume sample preps.
Run-to-Run Reproducibility
Every sample follows the same protocol to the same specification—no operator variation between technicians or across runs.
Throughput That Keeps Up
As capacity needs grow, Trilobot scales with them without the prep becoming the bottleneck.
End-to-End on One Platform
More of your workflow runs on one platform, so you spend less time managing instrument handoffs and more time on the science.
Flexible by Design
Swap tools, scale volumes, change formats—the Trilobot adapts to your workflow, not the other way around.
Starts at $25k/year
Full lab automation starting at $25k/year—less than most labs spend on a single liquid handler.
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Frequently asked
questions
Trilobio's NGS automation starts at $25k/year, well below traditional liquid handling vendors that charge hundreds of thousands of dollars. That cost includes the Trilobot for extraction and library prep, plus Trilobio OS for protocol design and run tracking. Because the Trilobot autocalibrates plasticware and switches tools without manual setup, labs also reclaim technician time previously spent babysitting runs. Combined, many labs see ROI in the range of 5x within the first year.
Trilobots automate the liquid-handling-intensive steps of extraction and library prep and orchestrate movements to key equipment, such as thermocyclers and plate readers. Your library comes off the Trilobot ready to be sequenced on your preferred platform, and the Trilobot API allows for native platform integration into your data ecosystem.
The Trilobot is compatible with library prep reagents from major kit manufacturers. Our protocols are designed to be flexible—if you're already running a specific kit, our team can work with you to automate your existing workflow rather than asking you to change it.
Yes. Trilobot supports both DNA (whole genome, targeted panel, etc.) and RNA (total RNA, mRNA, small RNA) library prep workflows. Protocol design is handled through Trilobio OS using biology-level steps, not code.
No. Protocols are designed in Trilobio OS using biology-level language: you define the steps in terms a biologist would recognize, not in machine code. Our team also provides onboarding support to get your specific protocol running quickly.