Platform
RobotToolsSoftwareEcosystem
Self-Driving LabsApplicationsCustomersBlogCompany
Talk to our applications team
Talk to our team

Next Generation Sequencing (NGS) Automation

From library prep to sample normalization, the Trilobot handles the repetitive, error-prone steps that stand between your samples and sequencing-ready plates.

Talk to our applications team

Scroll to discover

Automating NGS on Trilobots

A full NGS library prep runs 5-8 hours from input to sequencing-ready output. The Trilobot is built to automate your NGS workflow, scale throughput, and increase walkaway time. No babysitting required.

A diagram showing how Trilobio automates Next Gen Sequencing (NGS) workflows. Scale throughput and increase walkaway time, no babysitting required.

Start Small. Scale When You're Ready.

We get it—you may not need a full automation buildout on day one. Start with a single Trilobot, prove out your process, and expand when your throughput demands it—without rebuilding your protocol or your setup from scratch.

One Trilobot Three Trilobots Six Trilobots
Best for Labs getting into automation for the first time Labs seeking high walkaway time and increased throughput Labs optimizing for high-throughput operations
What it automates ~60% of your NGS workflow ~90% of your NGS workflow ~90% of your NGS workflow
Integrated equipment 1-2 instruments (e.g., thermocycler) 2-3 frames with multiple instruments 3+ frames with multiple instruments
Throughput 96+ samples/day 1000+ samples/day 2000+ samples/day
Walkaway time* 4 hours 7 hours 7 hours
Learn More
Learn More
Learn More
*Chart Based on an 8-hour workflow

At $25k/year, the Trilobot pays for itself

Most labs reach 5x ROI in year one, from technician time and failed plates alone.

Talk to our applications team

What is Next-Generation Sequencing?

Next-generation sequencing (NGS) is a massively parallel sequencing technology that generates high-resolution genomic data across entire genomes, transcriptomes, or targeted gene panels in a single run. It's used across academic research, pharma, and biotech to characterize genetic variation, profile gene expression, identify somatic mutations, and map epigenetic modifications, producing comprehensive data that manual sequencing methods can’t deliver at scale. The sequencing itself is the easy part. Library preparation—the fragmentation, end repair, adapter ligation, cleanup, and normalization steps that happen before your sample reaches the instrument—is where precision matters most and where manual pipetting introduces the most risk. A small volume error or an inconsistent bead cleanup can skew coverage, introduce artifacts, or invalidate samples entirely. That's the workflow Trilobio automates.

Challenges

Challenges of Manual NGS Library Prep

Equipment Babysitting

Multi-step prep requires constant attention across timed incubations, reagent additions, and plate transfers, keeping a technician tethered to the bench for most of the run.

High Cost Per Run

When a run fails, you're not just repeating bench work—you're re-spending on consumables, tracking down samples, and re-running analysis.

High Error Rate

Even small volume errors can skew data, introduce artifacts, or invalidate samples.

Low Throughput

Preparing samples manually can consume an entire day of technician time.

‍

Poor Sample-to-Sample Consistency

Variability between technicians and across runs adds noise to your data.

Opportunity Cost

Sample prep isn’t where the innovation happens, but it's often where scientists spend their time.

benefits

Benefits of Automating Next Gen Sequencing with Trilobio

Built-In Reliability

Consistent liquid handling at every step means data quality reflects your biology, not variability introduced at the bench.

Full Walkaway

Load your samples and walk away. Trilobot runs multi-step protocols unattended—no babysitting required.

More Samples, Less Time

The Trilobot runs unattended through multi-step protocols, reducing hands-on time across high-volume sample preps.

Run-to-Run Reproducibility

Every sample follows the same protocol to the same specification—no operator variation between technicians or across runs.

Throughput That Keeps Up

 As capacity needs grow, Trilobot scales with them without the prep becoming the bottleneck.

End-to-End on One Platform

More of your workflow runs on one platform, so you spend less time managing instrument handoffs and more time on the science.

Flexible by Design

Swap tools, scale volumes, change formats—the Trilobot adapts to your workflow, not the other way around.

Starts at $25k/year

Full lab automation starting at $25k/year—less than most labs spend on a single liquid handler.

Ready to automate Next Generation Sequencing in your lab?

Tell us about your sequencing workflow, and we'll show you exactly how the Trilobot fits in.
Talk to our applications team
Close-up of a black mechanical robotic arm component against a dark background.

Frequently asked
questions

Explore how it works, what makes it different, and how it transforms everyday biology workflows.

Talk to our team
How much does it cost to automate NGS?

Trilobio's NGS automation starts at $25k/year, well below traditional liquid handling vendors that charge hundreds of thousands of dollars. That cost includes the Trilobot for extraction and library prep, plus Trilobio OS for protocol design and run tracking. Because the Trilobot autocalibrates plasticware and switches tools without manual setup, labs also reclaim technician time previously spent babysitting runs. Combined, many labs see ROI in the range of 5x within the first year.

How does Next-Generation Sequencing work on Trilobots?

Trilobots automate the liquid-handling-intensive steps of extraction and library prep and orchestrate movements to key equipment, such as thermocyclers and plate readers. Your library comes off the Trilobot ready to be sequenced on your preferred platform, and the Trilobot API allows for native platform integration into your data ecosystem.

Which NGS library prep kits does Trilobio support?

The Trilobot is compatible with library prep reagents from major kit manufacturers. Our protocols are designed to be flexible—if you're already running a specific kit, our team can work with you to automate your existing workflow rather than asking you to change it.

Can Trilobot handle both DNA and RNA library prep?

Yes. Trilobot supports both DNA (whole genome, targeted panel, etc.) and RNA (total RNA, mRNA, small RNA) library prep workflows. Protocol design is handled through Trilobio OS using biology-level steps, not code.

Do I need to write custom software to set up my NGS protocol?

No. Protocols are designed in Trilobio OS using biology-level language: you define the steps in terms a biologist would recognize, not in machine code. Our team also provides onboarding support to get your specific protocol running quickly.

Powerful and reliable.
Full lab automation
you can afford.
Robot
Tools
Software
Ecosystem
Customers
Self-Driving Labs
Blog
Company
Talk to our applications team

By providing this information, you agree to be kept informed about Trilobio products and services.

Thank you! Your submission has been received!
Oops! Something went wrong while submitting the form.
Get the latest research insights, product updates, and application tips.
Privacy Policy
Terms of Service
© 2026 Trilobio. All Rights Reserved.
Made by tonik