Automated nucleic acid quantification & normalization
The Trilobot lays out the assay plate, orchestrates the plate read on integrated equipment, and normalizes every sample to target concentration.
Automating nucleic acid quantification & normalization on Trilobots
When it comes to automating nucleic acid quantification and normalization, the Trilobot lays out the assay plate, orchestrates the plate read, and normalizes every sample to target concentration in the same run. It's built to automate your nucleic acid quantification and normalization workflow, scale throughput, and increase walkaway time.

Nucleic acid quantification that scales with your lab
Every lab automates at a different pace. Start with one Trilobot handling plate prep, then connect a plate reader and scale into full extraction-through-normalization runs as your sample volume grows.
What is nucleic acid quantification & normalization?
Nucleic acid quantification measures how much DNA or RNA is in a sample. Normalization dilutes each one to the same target concentration before it moves to library prep, PCR, or cloning.
Challenges of manual nucleic acid quantification
Benefits of automating nucleic acid quantification with Trilobio
Built-In Reliability
Consistent liquid handling at every step means data quality reflects your biology, not variability introduced at the bench.
Full Walkaway
Load your samples and walk away. Trilobot runs multi-step protocols unattended—no babysitting required.
More Data Points Per Plate
Because the system is integratable with your assay setup, every well can run a different condition instead of duplicating one, giving you more usable data out of each plate.
Lower Contamination Risk
Every Trilobiot is enclosed and HEPA-filtered, keeping your samples sterile from start to finish.
End-to-End on One Platform
More of your workflow runs on one platform, so you spend less time managing instrument handoffs and more time on the science.
Run-to-Run Reproducibility
Every sample follows the same protocol to the same specification—no operator variation between technicians or across runs.
Flexible by Design
Swap tools, scale volumes, change formats—the Trilobot adapts to your workflow, not the other way around.
Starts at $25k/year
Full lab automation starting at $25k/year—less than most labs spend on a single liquid handler.
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Frequently asked
questions
Explore how nucleic acid quantification and normalization automation works, what makes it different with the Trilobot, and how Trilobio transforms everyday biology workflows.
You can automate nucleic acid quantification & normalization at as low as $25k/year with the Trilobot.
Most automated systems handle either the plate assay or the liquid handling, not both. The Trilobot lays out the assay plate, orchestrates the plate read, and calculates normalization dilutions in one connected run.
The Trilobot pipettes samples and quantification reagents into an assay plate, then hands the plate off to an integrated plate reader. Trilobio OS reads the concentration data and calculates the dilution volume each sample needs to reach the target concentration.
Nucleic acid normalization adjusts every sample in a batch to the same target DNA or RNA concentration, typically by diluting each one based on its measured concentration. It's a standard step before library prep, PCR, or cloning, where uneven starting concentrations produce inconsistent results downstream.
Downstream protocols like library prep and PCR are calibrated to a specific input amount, and a sample that's too concentrated or too dilute can throw off the reaction. Normalizing every sample to the same concentration before it moves forward keeps results comparable across the whole plate.