Platform
RobotToolsSoftwareEcosystem
Self-Driving LabsApplicationsCustomersBlogCompany
Talk to our applications team
Talk to our team

Automated nucleic acid quantification & normalization

The Trilobot lays out the assay plate, orchestrates the plate read on integrated equipment, and normalizes every sample to target concentration.

Talk to our applications team

Scroll to discover

Automating nucleic acid quantification & normalization on Trilobots

When it comes to automating nucleic acid quantification and normalization, the Trilobot lays out the assay plate, orchestrates the plate read, and normalizes every sample to target concentration in the same run. It's built to automate your nucleic acid quantification and normalization workflow, scale throughput, and increase walkaway time.

The automated nucleic acid quantification and normalization workflow performed on the Trilobot

Nucleic acid quantification that scales with your lab

Every lab automates at a different pace. Start with one Trilobot handling plate prep, then connect a plate reader and scale into full extraction-through-normalization runs as your sample volume grows.

One Trilobot One Trilobot One Trilobot
Best for Targeted solution for plate prep and general liquid handling Targeted solution for nucleic acid quantification Comprehensive solution for nucleic acid and NGS workflows
What it automates ~80% of your QC workflow ~100% of your extraction and QC workflows ~100% of your extraction, QC, and NGS workflows
Integrated equipment None Plate reader 1-3 frames with multiple instruments
Throughput 3000+ samples/day 3000+ samples/day 3000+ samples/day
Walkaway time* 50 minutes 1 hour 1+ hours
Learn More
Learn More
Learn More
Based on a 1-hour workflow

Starting at $25k/year, the Trilobot pays for itself

Traditional lab automation systems can run into six figures. Learn how you can automate nucleic acid quantification in your lab at a fraction of the cost.

Talk to our applications team

What is nucleic acid quantification & normalization?

Nucleic acid quantification measures how much DNA or RNA is in a sample. Normalization dilutes each one to the same target concentration before it moves to library prep, PCR, or cloning.

Challenges

Challenges of manual nucleic acid quantification

Waiting Between Steps

Between timed incubations, washes, and spin steps, there's plenty of downtime—but you still can't leave the bench. You're present for the whole run, productive for only part of it.

Equipment Babysitting

Multi-step prep requires constant attention across timed incubations, reagent additions, and plate transfers, keeping a technician tethered to the bench for most of the run.

Low Throughput

Preparing samples manually can consume an entire day of technician time.

‍

Poor Sample-to-Sample Consistency

Variability between technicians and across runs adds noise to your data.

Opportunity Cost

Sample prep isn’t where the innovation happens, but it's often where scientists spend their time.

benefits

Benefits of automating nucleic acid quantification with Trilobio

Built-In Reliability

Consistent liquid handling at every step means data quality reflects your biology, not variability introduced at the bench.

Full Walkaway

Load your samples and walk away. Trilobot runs multi-step protocols unattended—no babysitting required.

More Data Points Per Plate

Because the system is integratable with your assay setup, every well can run a different condition instead of duplicating one, giving you more usable data out of each plate.

Lower Contamination Risk

Every Trilobiot is enclosed and HEPA-filtered, keeping your samples sterile from start to finish.

End-to-End on One Platform

More of your workflow runs on one platform, so you spend less time managing instrument handoffs and more time on the science.

Run-to-Run Reproducibility

Every sample follows the same protocol to the same specification—no operator variation between technicians or across runs.

Flexible by Design

Swap tools, scale volumes, change formats—the Trilobot adapts to your workflow, not the other way around.

Starts at $25k/year

Full lab automation starting at $25k/year—less than most labs spend on a single liquid handler.

Ready to automate nucleic acid quantification & normalization in your lab?

Tell us about your workflow, and we'll show you exactly how the Trilobot fits in.
Talk to our applications team
Close-up of a black mechanical robotic arm component against a dark background.

Frequently asked
questions

Explore how nucleic acid quantification and normalization automation works, what makes it different with the Trilobot, and how Trilobio transforms everyday biology workflows.

Talk to our team
How much does an automated nucleic acid quantification & normalization machine cost?

You can automate nucleic acid quantification & normalization at as low as $25k/year with the Trilobot.

How does the Trilobot compare to other automated nucleic acid quantification and normalization systems?

Most automated systems handle either the plate assay or the liquid handling, not both. The Trilobot lays out the assay plate, orchestrates the plate read, and calculates normalization dilutions in one connected run.

How does Trilobio automate nucleic acid quantification?

The Trilobot pipettes samples and quantification reagents into an assay plate, then hands the plate off to an integrated plate reader. Trilobio OS reads the concentration data and calculates the dilution volume each sample needs to reach the target concentration.

What is nucleic acid normalization?

Nucleic acid normalization adjusts every sample in a batch to the same target DNA or RNA concentration, typically by diluting each one based on its measured concentration. It's a standard step before library prep, PCR, or cloning, where uneven starting concentrations produce inconsistent results downstream.

Why is nucleic acid normalization necessary?

Downstream protocols like library prep and PCR are calibrated to a specific input amount, and a sample that's too concentrated or too dilute can throw off the reaction. Normalizing every sample to the same concentration before it moves forward keeps results comparable across the whole plate.

Powerful and reliable.
Full lab automation
you can afford.
Robot
Tools
Software
Ecosystem
Customers
Self-Driving Labs
Blog
Company
Talk to our applications team

By providing this information, you agree to be kept informed about Trilobio products and services.

Thank you! Your submission has been received!
Oops! Something went wrong while submitting the form.
Get the latest research insights, product updates, and application tips.
Privacy Policy
Terms of Service
© 2026 Trilobio. All Rights Reserved.
Made by tonik